YILIMART - Pan DC MicroBeads, mouse

Pan DC MicroBeads, mouse

For research use only

货号：: 130-092-465
描述：: Pan DC MicroBeads, mouse
包装：: for 2×10⁹ total cells

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Pan DC MicroBeads consist of CD11c and Anti-mPDCA-1 MicroBeads. The combination of both, the CD11c and Anti-mPDCA-1 MicroBeads, together ensures the highest recovery and purity of all DCs in one isolation step.

Background information

While CD11c is expressed at high levels on classical dendritic cells (DCs), it is only expressed at low to intermediate levels on plasmacytoid dendritic cells (PDCs).

The mouse plasmacytoid dendritic cell antigen-1 (mPDCA-1) is specifically expressed on PDCs with the phenotype CD11c

^low

, CD45R(B220)

⁺

, and Ly-6C

⁺

in lymphoid and non-lymphoid tissues. Due to the low expression of CD11c on PDCs, using CD11c expression alone might not lead to enrichment of PDCs to the same extent as classical DCs.

Downstream applications

Mouse DCs isolated with Pan DC MicroBeads are suitable for phenotypical and functional analyses, molecular analyses, coculture experiments to evaluate their interaction with other cell types, migration experiments, or adoptive transfer.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Spleen cells before separation	Spleen cells depleted of DCs

Isolated total DCs

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