YILIMART - aCLA MB Kit, human

The Anti-CLA MicroBead Kit was developed for the positive selection or depletion of human CLA

⁺

cells from PBMCs, lymphoid and non-lymphoid tissue for phenotypical and functional characterization.

Background information

The cutaneous lymphocyte-associated antigen (CLA) is an inducible carbohydrate modification of P-selectin glycoprotein ligand 1 (PSGL-1) and functions as ligand for E-selectin. CLA is a skin-homing receptor and is found on a minor subset of human T cells that infiltrate the skin. This post-translational modification of PSGL-1 is discussed to serve as mechanism to regulate homing of CD4

⁺

and CD8

⁺

memory/effector T cells from peripheral blood to the skin; an immune response that plays an essential role during many inflammatory and certain malignant skin diseases.

In peripheral blood, CLA is expressed on skin-homing memory/effector T cells, on few B cells, NK cells, and almost all monocytes and dendritic cells, including Langerhans cells in the skin.

The Anti-CLA MicroBead Kit can be used in combination with MACS

^®

Cell Isolation Kits or MACS MultiSort Kits, for example, with the CD8

⁺

T Cell Isolation Kit for the isolation of CD8

⁺

CLA

⁺

memory/effector T cells.

Detailed separation procedure

The isolation is performed by a two-step procedure. The CLA

⁺

cells are fluorescently and indirectly magnetically labeled with Anti-CLA-PE and Anti-PE MicroBeads.

Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Isolation of CLA

⁺

cells from PBMCs using the Anti-CLA MicroBead Kit, a MiniMACS™ Separator, and an MS Column.

PBMCs before separation	CLA ^- cells

Isolated CLA ⁺ cells

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aCLA MB Kit, human

For research use only

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Background information

Detailed separation procedure

Columns

Figure 1

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