YILIMART - Anti-Prominin Microbead Kit, mouse

Anti-Prominin-1 MicroBeads have been developed for the isolation of mouse Prominin-1

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neural progenitor cells.

Background information

Prominin-1 is a glycoprotein with close structural similarity to its human ortholog, CD133

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. Prominin-1 expression has been demonstrated on cells of different embryonic and adult tissues, including ES-derived committed and early progenitor cells

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, the developing

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and postnatal

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central nervous system, kidney tubules

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, and cells of the hematopoietic system

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. In the developing nervous system prominin-1 is predominantly expressed on neuroepithelial cells and other epithelia. In the postnatal brain, prominin-1 expression has been found in areas of neurogenesis, such as the subventricular zone (SVZ). Prominin-1 is preferentially localized to plasma membrane protrusions of the apical membrane.

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Columns

For positive selection: MS, LS, or autoMACS

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Columns. For depletion: LD or autoMACS Columns.

Figure 1

Enrichment of Prominin-1

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cells from mouse cerebellum (postnatal day 1) using Anti-Prominin-1 MicroBeads, a MiniMACS™ Separator, and MS Columns.

Mouse cerebellar cells before separation	Prominin-1 ^– cells

Prominin-1 ⁺ cells

Selected references
1. Weigmann, A. et al. (1997) Prominin, a novel microvilli-specific polytopic membrane proteine of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells. Proc. Natl. Acad. Sci. U.S.A. 94: 12425-12430
2. Kania, G. et al. (2005) Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors. Stem Cells 23: 791-804
3. Corbeil, D. et al. (2001) Prominin: a story of cholesterol, plasma membrane protrusions and human pathology. Traffic 2: 82-91
4. Lee, A. et al. (2005) Isolation of neural stem cells from the postnatal cerebellum. Nat. Neurosci. 8: 723-729
5. Marzesco, A.-M. et al. (2005) Release of extracellular membrane particles carrying the stem cell marker prominin-1 (CD133) from neural progenitors and other epithelial cells. J. Cell. Sci. 118: 2849-2858
Brochures and posters

Neuroscience research
Scientific posters

Semi-automated tissue dissociation and preserved epitope integrity optimize immunomagnetic sorting of neural cells

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Anti-Prominin Microbead Kit, mouse

For research use only

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Background information

Columns

Figure 1

Selected references

Brochures and posters

Scientific posters

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