YILIMART - CD38 MicroBead Kit, human

The CD38 MicroBead Kit was developed for the positive isolation or depletion of CD38

⁺

cells from human PBMCs, bone marrow or tissue. The CD38

⁺

cells are indirectly magnetically labeled with CD38-Biotin and Anti-Biotin MicroBeads.

Background information

CD38 is a 45 kDa multi-lineage type II transmembrane glycoprotein. It is expressed at varying levels on the majority of hematopoietic as well as on some nonhematopoietic cells. The expression level depends on the developmental state and the activation status. CD38 is absent on most primitive CD34

⁺

stem cells and upregulated on CD34

⁺

cells upon lymphocyte commitment.

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Applications

The CD38 MicroBead Kit can be applied for positive selection or depletion of hematopoietic cell subsets expressing human CD38 antigen, such as CD38

⁺

B cells, T cells, and NK cells from PBMCs, bone marrow, or tissue. The kit is also suitable for the isolation of CD38

⁺

plasma cells from tissue, such as bone marrow.

The CD38 MicroBead Kit can also be used for depletion of CD38

⁺

cells to isolate CD38

^–

cell subsets, e.g., CD34

⁺

CD38

^–

stem cells in combination with the CD34 MicroBead Kit.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Depletion of CD38

⁺

cells from PBMCs using the CD38 MicroBead Kit, an LD Column, and a MidiMACS Separator. Cell fractions are fluorescently stained with Anti-Biotin-FITC and CD19-PE to detect cells labeled with the CD38 MicroBead Kit.

Unseparated fraction	PBMCs depleted of CD38 ⁺ cells

Selected references
1. Howard, M. et al. (1993) Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38. Science 262(5136): 1056-1059
2. Terstappen et al. (1991)
  
  Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34
  ⁺
  CD38
  ^–
  progenitor cells.
  
  Blood 77: 1218-1227
3. Deterre et al. (2000) CD38 in T- and B-cell functions. Chem. Immunol. 75: 146-168
4. Sconocchia et al. (1999) CD38 triggers cytotoxic responses in activated human natural killer cells. Blood 94: 3864-3871
5. Terstappen, L. W. et al. (1990) Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J. Leukoc. Biol. 48: 138-148
6. Takasawa, S. et al. (1993) Cyclic ADP-ribose in insulin secretion from pancreatic beta cells. Science 259: 370-373
7. Dianzani, U. et al. (1994)
  
  Interaction between endothelium and CD4
  ⁺
  CD45RA
  ⁺
  lymphocytes. Role of the human CD38 molecule.
  
  J. Immunol. 154: 952-959
8. Funaro, A. et al. (1990) Involvement of the multilineage CD38 molecule in a unique pathway of cell activation and proliferation. J. Immunol. 145: 2390-2396
9. Deaglio et al. (2002) Human CD38 and CD16 are functionally dependent and physically associated in natural killer cells. Blood 99: 2490-2498
10. Zilber, M. T. et al. (2000) CD38 expressed on human monocytes: a coaccessory molecule in the superantigen-induced proliferation. Proc. Natl. Acad. Sci. U.S.A. 97: 2840-2845

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CD38 MicroBead Kit, human

For research use only

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Background information

Applications

Columns

Figure 1

Selected references

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