YILIMART - Lineage Cell Depletion Kit, human

The Lineage Cell Depletion Kit is an indirect magnetic labeling system for the depletion of mature hematopoietic cells such as T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, erythroid cells, and their committed precursors from bone marrow aspirate, cord blood, or mobilized leukapheresis product. The depletion of lineage-committed cells results in the enrichment of untouched stem and progenitor cells.

Detailed separation procedure

Cells are first labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens (CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a (Glycophorin A)). These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells.

Downstream applications

Flow cytometric characterization of rare stem and progenitor cell subsets
Evaluation of hematopoietic differentiation potential in vitro using CFU assays (CFC, CFU-GM, BFU-E, and CFU-Mk)
Evaluation of the CFU-F potential

Columns

MS, LS, XS, or autoMACS

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Columns.

Figure 1

Isolation of untouched lineage-negative cells from bone marrow mononuclear cells (BM-MNCs) using the Lineage Cell Depletion Kit and an LS Column. Cells were fluorescently stained with CD34-FITC.

BM-MNCs before separation	Lineage ⁺ fraction

CD34 ⁺ cells in the lineage cell–depleted fraction.

Selected references
1. Mizokami, T. et al. (2009) Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells. Haematologica 94: 618-628
2. Schreiber, T. D. et al. (2009) The integrin alpha9beta1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation. Haematologica 94: 1493-1501

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Lineage Cell Depletion Kit, human

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Detailed separation procedure

Downstream applications

Columns

Figure 1

Selected references

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