YILIMART - CD4 T Cell Iso.Kit, non-human primate

CD4 T Cell Iso.Kit, non-human primate

For research use only

货号：: 130-092-144
描述：: CD4⁺ T Cell Isolation Kit, non-human primate
包装：: for 1×10⁹ total cells

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The CD4

⁺

T Cell Isolation Kit was developed for the isolation of untouched CD4

⁺

T cells from rhesus monkey PBMCs.

Background information

Non-CD4

⁺

T cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Highly pure untouched CD4

⁺

T cells are obtained by depletion of the magnetically labeled cells.

Untouched CD4

⁺

T cells may also be used for further isolation of specific T cell subsets. The CD4

⁺

CD25

⁺

Regulatory T Cell Isolation Kit is recommended for most convenient separation of CD4

⁺

CD25

⁺

regulatory T cells. Furthermore, the CD4

⁺

T Cell Isolation Kit can be used in combination with CD95-PE and Anti-PE MicroBeads to isolate naive CD4

⁺

T cells from non-human primate PBMCs. A special protocol for this application is available.

Downstream applications

T cells isolated with the CD4

⁺

T Cell Isolation Kit are used when direct labeling of T cell surface molecules could interfere with downstrem applications, e.g., in studies on T helper cell activation.

Columns

MS, LS, or autoMACS

^®

Columns.

Figure 1

Isolation of untouched CD4

⁺

T cells from rhesus monkey PBMCs using the CD4

⁺

T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells are fluorescently stained with CD45-FITC, CD4-PE and CD3-APC. Plots show viable CD45

⁺

leukocytes.

PBMCs before separation	Isolated CD4 ⁺ T cells

Special protocols

Isolation of naive CD4
⁺
T cells from non-human primate PBMCs

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