YILIMART - CD8 T Cell Iso.Kit, non-human primate

CD8 T Cell Iso.Kit, non-human primate

For research use only

货号：: 130-092-143
描述：: CD8⁺ T Cell Isolation Kit, non-human primate
包装：: for 1×10⁹ total cells

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The CD8

⁺

T Cell Isolation Kit was developed for the isolation of untouched CD8

⁺

T cells from rhesus monkey PBMCs.

Background information

Non-CD8

⁺

T cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Highly pure untouched CD8

⁺

T cells are obtained by depletion of the magnetically labeled cells.

Downstream applications

T cells isolated with the CD8

⁺

T Cell Isolation Kit are used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on cytotoxic T cell activation.

Untouched CD8

⁺

T cells may also be used for further isolation of specific cytotoxic T cell subsets.

Columns

MS, LS, or autoMACS

^®

Columns.

Figure 1

Isolation of untouched CD8

⁺

T cells from rhesus monkey PBMCs using the CD8

⁺

T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells are fluorescently stained with CD45-FITC, CD8-PE, and CD3-APC. Plots show viable CD45

⁺

leukocytes.

PBMCs before separation	Isolated CD8 ⁺ T cells

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