YILIMART - CD146 (LSEC) MicroBeads, mouse

The CD146 (LSEC) MicroBeads were developed for the positive selection or depletion of CD146

⁺

cells from mouse tissues.

Background information

CD146 is a transmembrane glycoprotein and belongs to the IgG superfamily of cell adhesion molecules.

¹

The CD146 (LSEC) antibody binds to mouse liver sinusoidal endothelial cells (LSECs) which are microvascular endothelial cells lining the hepatic sinusoidal wall and mainly contribute to the control of immune responses against circulating soluble antigens in the liver. Their strategic positioning favors a tight interaction with lymphocytes migrating through the liver. LSECs possess a high capacity for antigen uptake and processing but express, in contrast to professional antigen-presenting cells (e.g. dendritic cells), only low levels of costimulatory molecules.

²

The CD146 (LSEC) antibody also binds to endothelial cells from a various range of organs, such as skin, liver, kidney, brain, spleen, lymph node, intestine, heart and skeletal muscle as well as on blood vessel structures, such as pulmonary arteries, veins, and the capillary network of the alveolar walls but not on lymphatic endothelium. In contrast to humans that express CD146 also on T cells or follicular dendritic cells, murine CD146 expression was only found on a subset of NK1.1

⁺

cells at low levels.

³

Downstream applications

Endothelial cells from spleens, lungs, kidneys, and livers

³

as well as LSECs from liver

⁴

have been selected using the CD146 (LSEC) MicroBeads. Mouse LSECs isolated with CD146 (LSEC) MicroBeads are suitable for functional studies and co-culture experiments.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

LSECs were isolated from a mouse liver cell suspension using CD146 (LSEC) MicroBeads, an MS Column, and a MiniMACS™ Separator. The cells were stained with CD146 (LSEC)-FITC and CD49b (DX5)-APC.

Mouse liver cells before separation	Mouse liver cells depleted of LSECs

Selected references
1. Sers, C. et al. (1993) Genomic organization of the melanoma-associated glycoprotein MUC18: implications for the evolution of the immunoglobulin domains. Proc. Natl. Acad. Sci. U.S.A. 90: 8514-8518
2. Diehl, L. et al. (2008)
  
  Tolerogenic maturation of liver sinusoidal endothelial cells promotes B7-homolog 1-dependent CD8
  ⁺
  T cell tolerance.
  
  Hepatology 47: 296-305
3. Schrage, A. et al. (2008) Murine CD146 is widely expressed on endothelial cells and is recognized by the monoclonal antibody ME-9F1. Histochem. Cell Biol. 129: 441-451
4. Hegenbart et al. (2006) MACS&more 10: 8-10
Special protocols

Isolation of endothelial cells from mouse liver

查看数据文件查看下载

CD146 (LSEC) MicroBeads, mouse

For research use only

您浏览过的商品

Background information

Downstream applications

Columns

Figure 1

Selected references

Special protocols

相关产品