YILIMART - CD31 MicroBead Kit, human

The CD31 MicroBead Kit has been developed for the isolation of CD31

⁺

endothelial cells from single-cell suspensions.

Background information

CD31 is a glycoprotein, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1). It is expressed on mature endothelial cells, platelets, and on some white blood cells such as monocytes, NK cells, granulocytes, B cells, and T cell subsets.

Applications

The CD31 MicroBead Kit can be used for the isolation of human dermal vascular endothelial cells (HDMECs) from dermal tissues, for example foreskin, and human umbilical vein endothelial cells (HUVECs) from umbilical cord vein.

The CD31 MicroBead Kit has also been used for:

the isolation of endothelial cells from colorectal cancer and healthy colon tissue¹ ,
the enrichment of lymphangioma-derived lymphatic endothelial cells from fibroblast-contaminated cultures² ,
the isolation of human microvascular endothelial cells from dermal or renal tissue³ .

Columns

LS or autoMACS

^®

Columns.

Figure 1

Separation of CD31

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HDMECs from a preparation of dermal-layer cells of human foreskin using the CD31 MicroBead Kit and a MidiMACS™ Separator with an LS Column. Cells were fluorescently stained with CD31-APC.

HDMECs before separation	CD31 ^– cells

CD31 ⁺ cells

Figure 2
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Figure 2

Cultured HDMECs after extraction from foreskin tissue and purification using the CD31 MicroBead Kit.

Cultured HDMECs after extraction from foreskin tissue and purification using the CD31 MicroBead Kit.

Selected references
1. Schellerer, V. S. et al. (2007) Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture. Lab. Invest. 87: 1159-1170
2. Norgall et al. (2007) Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas. BMC Cancer 7: 105
3. Fonsato, V. et al. (2006) Expression of Pax2 in human renal tumor-derived endothelial cells sustains apoptosis resistance and angiogenesis. Am. J. Pathol. 168: 706-713
Special protocols

Isolation of human dermal microvascular endothelial cells (HDMECs) from foreskin tissue

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CD31 MicroBead Kit, human

For research use only

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Background information

Applications

Columns

Figure 1

Figure 2

Figure 2

Selected references

Special protocols

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