Background information

Downstream applications

Columns

• Figure 1

  Separation of non-mobilized PBMCs using CD133/1 (AC133)-Biotin, Anti-Biotin MicroBeads, MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with CD34-FITC and CD133/2 (293C3)-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

  Unseparated fraction	CD133+ cells

• Selected references

  1. Galic, Z. et al. (2006) T lineage differentiation from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 103: 11742-11747
  2. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
  3. Piechaczek, C. (2001) CD133. J. Biol. Regul. Homeost. Agents 15: 101-102
  4. Gallacher, L. et al. (2000)
     Isolation and characterization of human CD34

     ^–

     Lin

     ^–

     and CD34

     ⁺

     Lin

     ^–

     hematopoietic stem cells using cell surface markers AC133 and CD7.
     Blood 95(ARVO Annual Meeting Abstract): 2813-2820
  5. Bühring, H. J. et al. (1999) Expression of novel surface antigens on early hematopoietic cells. Ann. N. Y. Acad. Sci. 872: 25-39
  6. Gehling, U. M. et al. (2000)
     In vitro

     differentiation of endothelial cells from AC133-positive progenitor cells.
     Blood 95: 3106-3112
  7. Peichev, M. et al. (2000)
     Expression of VEGFR-2 and AC133 by circulating human CD34

     ⁺

     cells identifies a population of functional endothelial precursors.
     Blood 95: 952-958
  8. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
  9. Cummings, B. J. et al. (2005) Human neural stem cells differentiate and promote locometer recovery in spinal cord-injured mice. Proc. Natl. Acad. Sci. U.S.A. 102: 14069-14074
  10. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
  11. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
  12. Thill, M. et al. (2004)
      Identification of a population of CD133

      ⁺

      precursor cells in the stroma of human cornea.
      Invest. Ophthalmol. Vis. Sci. 45: 3519
  13. Stamm, C. et al. (2003) Autologous bone-marrow stem-cell transplantation for myocardial regeneration. Lancet 4, 361(9351): 45-46
  14. Stamm, C. et al. (2007)
      Intramyocardial delivery of CD133

      ⁺

      bone marrow cells and coronary artery bypass grafting for chronic ischemic heart disease: safety and efficacy studies.
      J. Thorac. Cardiovasc. Surg. 133: 717-725
  15. Klein (2007) Eur. Cardiovasc. Dis. 1: 123-125
  16. Klein, H. M. et al. (2007)
      Intramyocardial implantation of CD133

      ⁺

      stem cells improved cardiac function without bypass surgery.
      Heart Surg. Forum 10: E66-69
  17. Bartunek et al. (2005) Circulation 30: 178-183
  18. am Esch, J. S. 2nd et al. (2005)
      Portal application of autologous CD133

      ⁺

      bone marrow cells to the liver: a novel concept to support hepatic regeneration.
      Stem Cells 23(4): 463-470
  19. Fürst, G. et al. (2007)
      Portal vein embolization and autologous CD133

      ⁺

      bone marrow stem cells for liver regeneration: initial experience.
      J. Immunother. 243(1): 171-179
  20. Cañizo et al. (2007)
      Peripheral endothelial progenitor cells (CD133

      ⁺

      ) for therapeutic vasculogenesis in a patient with critical limb ischemia. One year follow-up.
      Cytotherapy 9(1): 99-102
  21. Kuçi et al. (2003)
      Identification of a novel class of human adherent CD34

      ^–

      stem cells that give rise to SCID-repopulating cells.
      Blood 101: 869-876
  22. Kuçi et al. (2008)
      Efficient

      in vitro

      generation of adult multipotent cells from mobilized peripheral blood CD133

      ⁺

      cells.
      Cell Prolif. 41: 12-27
  23. Kuçi et al. (2003) MACS&more 7(1): 6-8
  24. Kuçi et al. (2004) 2nd Intl. Meeting, Stem Cell Network, North-Rhine Westphalia. : abstract
  25. Hao, H. N. et al. (2003)
      Fetal human hematopoietic stem cells can differentiate sequentially into neural stem cells and then astrocytes

      in vitro

      .
      J. Hematother. Stem Cell Res. 12: 23-32
  26. Jang, Y. K. et al. (2004) Retinoic acid-mediated induction of neurons and glial cells from human umbilical cord-derived hematopoietic stem cells. J. Neurosci. Res. 75: 573-584
  27. Padovan et al. (2003) Expression of neuronal markers in differentiated marrow stromal cells and CD133+ stem-like cells. Cell Transplant 12: 839-848
  28. Piechaczek, C. et al. (2002)
      Differentiation of adult CD133

      ⁺

      cells isolated from peripheral blood into cells with a neural phenotype.
      Stem cell research (customer reports published by Miltenyi Biotec) : 2-3
  29. Belicchi, M. et al. (2004) Human skin-derived stem cells migrate throughout forebrain and differentiate into astrocytes after injection into adult mouse brain. J. Neurosci. Res. 77: 475-486
  30. Tamaki, S. et al. (2002) Engraftment of sorted/expanded human central nervous system stem cells from fetal brain. J. Neurol. Res. 69: 976-986
  31. Kelly, S. et al. (2004) Transplanted human fetal neural stem cells survive, migrate, and differentiate in ischemic rat cerebral cortex. Proc. Natl. Acad. Sci. U.S.A. 101: 11839-11844
  32. Yu, S. et al. (2004)
      Isolation and characterization of the CD133

      ⁺

      precursors from the ventricular zone of human fetal brain by magnetic affinity cell sorting.
      Biotechnol. Lett. 26: 1131-1136
  33. de Wynter, E. A. et al. (1998)
      CD34

      ⁺

      AC133

      ⁺

      cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
      Stem Cells 16: 387-396