YILIMART - CD117 MicroBead Kit, human

The CD117 MicroBead Kit has been developed for the positive selection of human hematopoietic stem and progenitor cells from peripheral blood, cord blood or bone marrow. It has also been used for the isolation of various stem cells from adult tissues, e.g. amniotic fluid stem cells

¹

and cardiomyocyte progenitors

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.

Background information

CD117 (also known as c-kit or stem cell factor receptor) is a receptor tyrosine kinase involved in cell signaling, activation and proliferation. CD117 is expressed on 1–3% of peripheral blood mononuclear cells and cord blood cells, and on up to 10% of bone marrow cells.

For flow cytometric analysis of separation results, the use of CD117 (A3C6E2) is recommended.

Downstream applications

The MicroBead-conjugated CD117 antibody (clone AC126) does not interfere with stem cell factor (SCF)-binding. Thus, CD117

⁺

cells isolated with the CD117 MicroBead Kit remain responsive to stimulation with the cytokine stem cell factor (SCF).

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Figure 1

Isolation of CD117

⁺

cells from bone marrow mononuclear cells (BM-MNCs) using the CD117 MicroBead Kit, a MiniMACS™ Separator and an MS Column. Cells were fluorescently stained with CD117 (A3C6E2)-PE and CD133/2 (293C3)-APC.

A:	B:
BM-MNCs before separation	CD117 ^- cells

C:
CD117 ⁺ cells

Selected references
1. De Coppi, P. et al. (2007) Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotechnol. 25: 100-106
2. Kubo, H. et al. (2008) Increased cardiac myocyte progenitors in failing human hearts. Circulation 118: 649-657

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CD117 MicroBead Kit, human

For research use only

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Background information

Downstream applications

Columns

Figure 1

Selected references

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