The Large Scale IFN-γ Secretion Assay - Enrichment Kit is suitable for the enrichment of human IFN-γ-secreting, antigen-specific T cells from leukapheresis harvests, PBMCs, or other leukocyte-containing single-cell preparations. Different from the standard Cytokine Secretion Assays, this assay uses a direct strategy for the magnetic labeling of the target cells.
Background information
The Large Scale IFN-γ Secretion Assay Enrichment Kit is exclusively designed for research use. Nevertheless, it can be used in combination with the manual cell separation systems as well as the autoMACS® Separator, the autoMACS® Pro Separator, and the CliniMACS® Plus Instrument.The MicroBeads are directly conjugated to a secondary IFN-γ-specific antibody (see figure 1). The kit includes Anti-IFN-γ-PE staining antibodies for flow cytometric analysis.
Applications
The Large Scale IFN-γ Secretion Assay - Enrichment Kit allows the concomitant enrichment of viable CD4- and CD8-positive IFN-γ secreting T cells, e.g., for the large-scale generation of antigen-specific cell lines or clones. Tedious subcloning is not necessary and both CD4
T-cell lines for adoptive transfer into recipients of allogeneic stem cell transplants.
Blood 103(9): 3565-3572
Feuchtinger, T. et al. (2006) Safe adoptive transfer of virus-specific T-cell immunity for the treatment of systemic adenovirus infection after allogeneic stem cell transplantation. Br. J. Haematol. 134(1): 64-76
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