YILIMART - CD4 Dendritic Cell Isol.Kit, mouse

CD4 Dendritic Cell Isol.Kit, mouse

For research use only

货号：: 130-091-262
描述：: CD4⁺ Dendritic Cell Isolation Kit, mouse
包装：: for 2×10⁹ total cells

- +

添加到购物车

您浏览过的商品

The CD4

⁺

Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4

⁺

DCs from single-cell suspensions of splenocytes.

Background information

CD4

⁺

DCs coexpress CD11c, CD11b, MHC class II, CD40, CD80, CD86 and are negative for CD8 and CD205. They represent the major population of DCs in spleen and comprise only a small population in peripheral lymph nodes (56% and 4% of all CD11c

⁺

DCs, respectively). The isolation of CD4

⁺

DCs is performed in two steps.

Detailed separation procedure

First, DCs are enriched by depletion of T cells, NK cells, B cells, and granulocytes with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Subsequently, CD4

⁺

DCs are positively selected from the enriched DC fraction with CD4 MicroBeads.

Downstream applications

CD4

⁺

DCs isolated using the CD4

⁺

Dendritic Cell Isolation Kit are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T cell polarization by CD4

⁺

DCs.