Background information

Downstream applications

Columns

• Figure 1

  Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE.

  A:	B:
  Before enrichment	Isolated CD117 + cells after enrichment

• Selected references

  1. Verda, L. et al. (2008) Hematopoietic mixed chimerism derived from allogeneic embryonic stem cells prevents autoimmune diabetes mellitus in NOD mice. Stem Cells 26: 381-386
  2. De Coppi, P. et al. (2007) Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotechnol. 25: 100-106
  3. Shin, J.Y. et al. (2014) High c-Kit expression identifies hematopoietic stem cells with impaired self-renewal and megakaryocytic bias J. Exp. Med. 211(2): 217-231
  4. Orlic, D. et al. (2001) Mobilized bone marrow cells repair the infarcted heart, improving function and survival. Proc. Natl. Acad. Sci. U.S.A. 98: 10344-10349