Background information

Downstream applications

Columns

• Figure 1

  BALB/c mice were immunized and the spleen cells restimulated with keyhole limpet hemocyanin (KLH) as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 233 IL-5-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only six IL-5-secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment.

  Stimulated sample

  Before enrichment	After enrichment
  Unstimulated control

  Before enrichment	After enrichment
  * Percentage represents frequency among CD4 + T cells.
  ** Percentage represents frequency among enriched cells.