Detailed separation procedure

Downstream applications

Columns

• Figure 1

  Untouched B cells were isolated from PBMCs using the B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator.

  Unseparated fraction	Untouched isolated B cells

• Figure 2

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  Figure 2

  Scanning electron micrograph of a B cell isolated with the B Cell Isolation Kit.

  (Courtesy of Prof. Groscurth, Zürich, Switzerland).

  
  Scanning electron micrograph of a B cell isolated with the B Cell Isolation Kit.

  (Courtesy of Prof. Groscurth, Zürich, Switzerland).

• Figure 3

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  Figure 3

  Isolation of untouched B cells using the B Cell Isolation Kit II.

  
  Isolation of untouched B cells using the B Cell Isolation Kit II.

• Selected references

  1. Poeck, H. et al. (2004) Plasmacytoid dendritic cells, antigen, and CpG-C license human B cells for plasma cell differentiation and immunoglobulin production in the absence of T-cell help. Blood 103: 3058-3064
  2. Marinkovic, D. et al. (2004) Identification of novel Myc target genes with a potential role in lymphomagenesis. Nucleic Acids Res. 32: 5368-5378
  3. Jaimes, M. C. (2004) Maturation and trafficking markers on rotavirus-specific B cells during acute infection and convalescence in children. J. Virol. 78: 10967-10976