YILIMART - CD20 MicroBeads, human

CD20 MicroBeads were developed for positive selection or depletion of human CD20

⁺

B cells from different cell sources such as human PBMCs, bone marrow, cell cultures or lymphoid tissues.

Background information

CD20 is a non-glycosylated transmembrane protein of 33–37 kDa that becomes heavily phosphorylated upon activation

^1,2

. It is expressed exclusively on B cells, including pre-B cells, naive, mature, and activated B cells, B cell blasts, and the majority of malignant B cells, but not on early B cell progenitors or plasma cells

^2,3

.

Downstream applications

Purified CD20

⁺

B cells may be used in cell biological studies, such as analysis of signal transduction, chemotaxis, allergen response, apoptosis, or in molecular studies, e.g., subset-specific microarrays.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

B cells were separated from PBMCs using CD20 MicroBeads and the autoMACS™ Separator.

PBMCs before separation	Enriched CD20 ⁺ cells

Selected references
1. Chang et al. (1996) Appl. Immunohistochem. 4: 1-15
2. Polyak et al. (2002) CD20 Workshop Panel report. In: Leucocyte typing VII, Oxford University Press.
3. Countouriotis, A. et al. (2002) Cell surface antigen and molecular targeting in the treatment of hematologic malignancies. Stem Cells 20: 215-229
4. Cragg, M. S. et al. (2002) Opposing properties of CD20 mAb. In: Leucocyte typing VII, Oxford University Press.

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CD20 MicroBeads, human

For research use only

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Background information

Downstream applications

Columns

Figure 1

Selected references

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