YILIMART - CD25 MicroBead Kit, mouse

CD25 MicroBead Kit, mouse

For research use only

货号：: 130-091-072
描述：: CD25 MicroBead Kit, mouse
包装：: for 1×10⁹ total cells

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The CD25 MicroBead Kit has been developed for the positive selection or depletion of mouse CD25

⁺

cells from single-cell suspensions of spleen and lymph nodes.

Background information

For separation, CD25

⁺

cells are indirectly magnetically labeled with CD25-PE and Anti-PE MicroBeads. CD25 is the IL-2R α-chain, which combined with the β-chain (CD122) and the γ-chain (CD132) forms the functional IL-2R complex. Binding of CD25-PE does not inhibit the adhesion of IL-2 to its receptor. IL-2Rα is expressed on activated T and B cells, to a lower extent on activated dendritic cells, on regulatory T cells, in the early stage of thymic T cell development (CD4

CD8

), and transiently during B cell ontogeny. NK cells and naive T and B cells do not express IL2-Rα.

Downstream applications

CD25

⁺

cells isolated by using the CD25 MicroBead Kit are suitable for

in vitro

analysis, coculture experiments, or adoptive transfer.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Isolation of CD25

⁺

cells from a mouse spleen cell suspension using the CD25 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator.

Spleen cells before separation	CD25 ^- cells after separation

CD25 ⁺ cells after separation

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