YILIMART - B Cell Isolation Kit, mouse

The B Cell Isolation Kit was developed for the fast and efficient isolation untouched resting B cells from single-cell suspensions of lymphoid tissues. The separation protocol is faster now! Download the updated datasheet.

Detailed separation procedure

Highly pure resting B cells are isolated by magnetic labeling and depletion of CD43-expressing B cells (activated B cells, plasma cells and CD5

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B-1a cells) and non-B cells. CD43

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B cells, T cells, NK cells, dendritic cells, macrophages, granulocytes, and erythroid cells are labeled with a cocktail of biotin-conjugated antibodies against CD43 (Ly-48), CD4 (L3T4), and Ter-119, as well as Anti-Biotin MicroBeads. The B Cell Isolation Kit includes the Biotin-Antibody Cocktail for labeling of non-target cells and Anti-Biotin MicroBeads.

Downstream applications

Untouched B cells may be used for further magnetic separation of B cells into subpopulations. B cells isolated with the B Cell Isolation Kit were e.g. analyzed for transgene expression by Northern blot analysis in a transgenic reporter model.

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Columns

MS, LS, or autoMACS

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Figure 1

Isolation of untouched B cells from a mouse spleen single-cell suspension using the B Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column.

Unseparated fraction	Isolated CD45R ⁺ B cells

Selected references
1. Harrow, F. and Ortiz, B.D. (2005) The TCRalpha locus control region specifies thymic, but not peripheral, patterns of TCRalpha gene expression. J. Immunol. 175: 6659-6667

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B Cell Isolation Kit, mouse

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Detailed separation procedure

Downstream applications

Columns

Figure 1

Selected references

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