YILIMART - Anti-PE MultiSort Kit

The Anti-Phycoerythrin (PE) MultiSort Kit provides a flexible tool for the sorting of cells according to multiple surface markers. The bright fluorescence intensity of PE-conjugated antibodies makes the Anti-PE MultiSort Kit especially useful for separation and subsequent detection of cells with weakly expressed markers.

Detailed separation procedure

Cells are first stained with a MACS

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PE-conjugated primary antibody and subsequently magnetically labeled with Anti-PE MultiSort MicroBeads. Following positive selection, the magnetic particles are removed from the cells by using the MultiSort Release Reagent. The cell population can then be magnetically labeled and sorted using a second marker of interest. This is achieved by labeling the cells directly or indirectly with MACS

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MicroBeads.

Applications

The Anti-PE MultiSort Kit is recommended if a positive selection according to two cell surface antigens is desired. The kit can, for example, be used for the positive selection of rodent T cell, B cell, or progenitor cell subsets.

Columns

MS, LS, XS, or autoMACS

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Columns.

Figure 1

Separation of human CD3

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CD56

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cells from PBMCs using CD56-PE, the Anti-PE MultiSort Kit, and an MS Column for the first positive selection as well as CD3 MicroBeads and an MS Column for the second positive selection.

PBMCs before separation	Isolated CD56 ⁺ cells

Isolated CD3 ⁺ CD56 ⁺ cells

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Anti-PE MultiSort Kit

For research use only

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Detailed separation procedure

Applications

Columns

Figure 1

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