YILIMART - Anti-DC (OX62) MicroBeads, rat

Anti-DC (OX62) MicroBeads have been developed for the positive selection or depletion of OX62

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dendritic cells (DCs) from blood, lymphoid, and non-lymphoid tissue.

Background information

The monoclonal antibody OX62 recognizes the integrin αE2 subunit (molecular mass: 150 kDa). OX62 is expressed on the

CD4

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and the CD4

^-

subset of dendritic cells but not on plasmacytoid dendritic cells in lymphoid tissue or on epidermal Langerhans cells. OX62 is also found on a subpopulation of epidermal TCRα/β

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or TCRγ/δ

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T cells and on a subset of folliculo-stellate-like (S100

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) cells in the anterior pituitary. It is not expressed on macrophages.

Downstream applications

Isolated OX62

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dendritic cells were used, for example, for analysis of the differential phenotype, function, and migration of dendritic cell subsets.

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Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Isolation of dendritic cells from rat spleen using Anti-DC (OX62) MicroBeads, a MiniMACS™ Separator, and an MS Column. Isolated cells were stained with Anti-MHC class II (OX6)-FITC, CD4-PE, and Anti-DC (OX62)-APC.

A:	B:
Spleen cells before separation	Isolated dendritic cells

C:
Isolated dendritic cells

Figure 2
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Figure 2

Isolated OX62
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dendritic cells from rat spleen, May-Grünwald/Giemsa stained. (Courtesy of Dr. R. Josien, Nantes, France.)

Isolated OX62
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dendritic cells from rat spleen, May-Grünwald/Giemsa stained. (Courtesy of Dr. R. Josien, Nantes, France.)

Selected references
1. Kobayashi, H. et al. (2004)
  In situ
  demonstration of dendritic cell migration from rat intestine to mesenteric lymph nodes: relationships to maturation and role of chemokines.
  
  J. Leukoc. Biol. 75: 434-442

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Anti-DC (OX62) MicroBeads, rat

For research use only

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Background information

Downstream applications

Columns

Figure 1

Figure 2

Figure 2

Selected references

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