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• Figure 1

  BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 236 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 48 IL-4-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells.

  Stimulated sample

  Before enrichment	After enrichment
  Unstimulated control

  Before enrichment	After enrichment
  * Percentage represents frequency among CD4 + T cells.

  ** Percentage represents frequency among enriched cells.

• Selected references

  1. Ouyang, W. et al. (2000) Stat6-independent GATA-3 autoactivation directs IL-4-independent Th2 development and commitment. Immunity 12: 27-37
  2. Hu-Li, J. et al. (2001) Regulation of expression of IL-4 alleles: analysis using a chimeric GFP/IL-4 gene. Immunity 14: 1-11
  3. Löhning, M. et al. (2003) Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation. Proc. Natl. Acad. Sci. U.S.A. 100: 12307-12312
  4. Guo, L. et al. (2004) Probabilistic regulation of IL-4 production in Tʜ2 cells: accessibility at the Il4 locus. Immunity 20: 193-203
  5. Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153