YILIMART - Anti-Biotin MicroBeads

Anti-Biotin MicroBeads have been developed for indirect magnetic labeling and separation of cells or other biological materials that are labeled with a biotinylated primary antibody or ligand, or with a cocktail of biotinylated antibodies. When working with debris-rich starting material and low frequency samples we recommend Anti-Biotin MicroBeads UltraPure to benefit from high specific enrichment with low background of debris and dead cells.

Background information

Anti-Biotin MicroBeads UltraPure have been especially developed for highly efficient separation of cells from debris-rich starting material. The biotinylated molecule is recognized by a monoclonal anti-biotin antibody coupled to MicroBeads. Anti-Biotin MicroBeads and Anti-Biotin MicroBeads UltraPure have the advantage of not binding to free biotin, which is often present in culture media. This feature provides the most sensitive labeling and separation of cells. With UltraPure MicroBeads we offer an ideal solution for ultrapure cell separation of cells even from debris-rich samples or other low frequency biological starting materials.

Applications

Anti-Biotin MicroBeads are ideal for positive selection, even of cells with low antigen expression, or for depletion. Anti-Biotin MicroBeads have been used for the separation of murine α

_E ^-

CD25

⁺

and α

_E ^-

CD25

^-

T cell subsets

¹

and for the isolation of CD45.2

⁺

cells from the bone marrow of chimeric mice

²

. They have also been used for the enrichment of human CD94

⁺

NK cells

³

. Anti-Biotin MicroBeads UltraPure have been especially designed for high-efficient cell isolation even from debris-rich samples and starting materials with increased amount of dead cells.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Separation of CD3

⁺

cells from human PBMCs using a biotinylated CD3 antibody, Anti-Biotin MicroBeads, and an MS Column.

PBMCs before separation	CD3 ^- cells

CD3 ⁺ cells

Figure 2

Human CLEC9a

⁺

cells were isolated from a debris-rich sample of peripheral blood mononuclear cells (PBMCs) using Anti-CLEC9a- Biotin, Anti-Biotin MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-Biotin-PE (# 130-090-756), CD141 (BDCA-3)-APC (# 130-090-907), and Propidium Iodide Solution (# 130-093-233). Cells were analyzed after gating on viable lymphoid cells.

Before separation	After separation

Selected references
1. Lehmann, J. et al. (2002) Expression of the integrin alpha Ebeta 7 identifies unique subsets of CD25+ as well as CD25- regulatory T cells. Proc. Natl. Acad. Sci. U.S.A. 99: 13031-13036
2. Lang, R. et al. (2003) SOCS3 regulates the plasticity of gp130 signaling. Nat. Immunol. 4: 546-550
3. Gastpar, R. et al. (2004) The cell surface-localized heat shock protein 70 epitope TKD induces migration and cytolytic activity selectively in human NK cells. J. Immunol. 172: 972-980
Customer reports and application notes

Magnetic enrichment of antigen-specific CD4
⁺
T cells enables the in-depth characterization of vaccine-induce dcirculating follicular T helper cells. Bergmann-Leitner, E. et al. (2016)

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Anti-Biotin MicroBeads

For research use only

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Background information

Applications

Columns

Figure 1

Figure 2

Selected references

Customer reports and application notes

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