Anti-ErbB-2 MicroBeads can be used for the enrichment of disseminated epithelial ErbB-2-expressing tumor cells from blood, bone marrow, or leukapheresis harvest.
Background information
ErbB-2, also known as HER2/neu, CD340 or p185, encodes the human epidermal growth factor receptor which is overexpressed in 25–30% of breast cancer patients as well as in various other tumors, e.g. ovarian, prostate, lung, colorectal, pancreatic, and gastric cancers.
Downstream applications
Enriched ErbB-2
+
cells can be characterized by using different techniques such as quantitative real-time PCR
1
, immunocytochemistry, or flow cytometry. The enriched cells can also be cultured.
A cluster of cytokeratin-positive cells isolated with Anti-ErbB-2 MicroBeads from peripheral blood of a patient with primary breast cancer. Cells were stained following the in-column intracellular staining protocol using the Inside Stain Kit in combination with Anti-Cytokeratin-Alkaline Phosphatase.
A cluster of cytokeratin-positive cells isolated with Anti-ErbB-2 MicroBeads from peripheral blood of a patient with primary breast cancer. Cells were stained following the in-column intracellular staining protocol using the Inside Stain Kit in combination with Anti-Cytokeratin-Alkaline Phosphatase.
Selected references
Molloy, T. J. et al. (2008) Towards an optimized platform for the detection, enrichment, and semi-quantitation circulating tumor cells. Breast Cancer Res. Treat. 112: 297-307
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