Anti-Melanoma (MCSP) MicroBeads were developed for
enrichment of disseminated melanoma cells from peripheral blood1 , bone marrow, leukapheresis harvest, and lymphoid tissue of melanoma patients – even in early disease stages2,3 ,
isolation of melanoma cells from single-cell suspensions of primary tumor tissue or primary skin cell culture.
Anti-Melanoma MicroBeads can be used in combination with the Inside Stain Kit. This enables the in-column staining of enriched melanoma cells for intracellular antigens, e. g., Melan-A. By performing combined enrichment and staining, a sensitivity of melanoma cell detection of one melanoma cell in 2×10
7
PBMC background cells can be achieved
4
. For convenient subsequent immunocytochemical detection the Melanoma Enrichment and Detection Kit can be used.
Background information
The antibody clone 9.2.27 recognizes the melanoma-associated chondroitin sulfate proteoglycan (MCSP) antigen, also known as high molecular weight melanoma-associated antigen, or NG2. MCSP is expressed on melanoma cells but not on carcinoma cells, fibroblastoid cells, or cells of hematopoietic origin.
Downstream applications
Cells enriched with Anti-Melanoma (MCSP) MicroBeads can be analyzed by immunocytochemistry, flow cytometry, or molecular biology methods. The isolated cells can also be cultured.
Melanoma cells from peripheral blood of a malignant melanoma patient isolated with Anti-Melanoma (MCSP) MicroBeads. Cells were stained according to the in-column, intracellular staining protocol using the Inside Stain Kit in combination with an anti-Melan A antibody and alkaline phosphatase.
Melanoma cells from peripheral blood of a malignant melanoma patient isolated with Anti-Melanoma (MCSP) MicroBeads. Cells were stained according to the in-column, intracellular staining protocol using the Inside Stain Kit in combination with an anti-Melan A antibody and alkaline phosphatase.
Selected references
Benez, A. et al. (1999) Detection of circulating melanoma cells by immunomagnetic cell sorting. J. Clin. Lab. Anal. 13: 229-233
Siewert, C. et al. (2001) Rapid enrichment and detection of melanoma cells from peripheral blood mononuclear cells by a new assay combining immunomagnetic cell sorting and immunocytochemical staining. Recent Results Cancer Res. 158: 51-60
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