Detailed separation procedure

Downstream applications

Columns

• Figure 1

  CD34 + HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34 + cells from PBMCs was followed by the depletion of CD34 + HLA-DR + cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34 + HLA-DR – cells (red).

  A:	B:
  PBMCs before separation	CD34 + cells
  C:
  CD34 + HLA-DR - cells

• Figure 2

  View details

  Figure 2

  Step-by-step CD34 MultiSort separation procedure.

  
  Step-by-step CD34 MultiSort separation procedure.

• Figure 3

  View details

  Figure 3

  CD34

  ⁺

  lin

  ^-

  cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)

  
  CD34

  ⁺

  lin

  ^-

  cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)

• Selected references

  1. Vodoyanik, M. A. et al. (2006) Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures. Blood 108: 2095-2105

• Special protocols

  Isolation of CD34

  ⁺

  HLA-DR

  ^-

  cells