YILIMART - CD138 MicroBeads, human

CD138 MicroBeads were developed for the positive selection or depletion of plasma cells from PBMCs, bone marrow, leukapheresis harvests, and single-cell suspensions from lymphoid tissues.

Background information

The CD138 antigen, also known as syndecan-1, is primarily expressed on normal and malignant plasma cells in the bone marrow. It is further found on a subset of plasma cells in peripheral blood and certain lymphoid tissues. The CD138 antigen is neither expressed on naive B cells, germinal center B cells, memory B cells, nor on T cells or monocytes.

Downstream applications

Since the direct study of plasma cells is hampered by their low frequency, prior enrichment of plasma cells using CD138 MicroBeads allows more accurate cell analysis, e.g. by flow cytometry

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. To ease the analysis of enriched plasma cells, CD138 MicroBeads are also available in combination with CD138-PE. Plasma cells purified with CD138 MicroBeads are applied for molecular biological studies, such as PCR

^1,4,7,8

, microarrays

^2,4,5,6

, or protein analysis

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.

Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

CD138

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plasma cells were isolated from normal PBMCs using CD138 MicroBeads, an MS Column, and a MiniMACS™ Separator.

PBMCs before separation	Enriched CD138 ⁺ cells

Selected references
1. Draube, A. et al. (2001) Immunomagnetic enrichment of CD138 positive cells from weakly infiltrated myeloma patients samples enables the determination of the tumor clone specific IgH rearrangement. Ann. Hematol. 80: 83-89
2. Shaughnessy, J. Jr. et al. (2001) Cyclin D3 at 6p21 is dysregulated by recurrent chromosomal translocations to immunoglobulin loci in multiple myeloma. Blood 98: 217-223
3. Horst, A. et al. (2002)
  
  Detection and characterization of plasma cells in peripheral blood: correlation of IgE
  ⁺
  plasma cell frequency with IgE serum titre.
  
  Clin. Exp. Immunol. 130: 370-378
4. Davies, F. E. et al. (2003) Insights into the multistep transformation of MGUS to myeloma using microarray expression analysis. Blood 102: 4504-4511
5. Tarte, K. et al. (2003) Gene expression profiling of plasma cells and plasmablasts: toward a better understanding of the late stages of B-cell differentiation. Blood 102: 592-600
6. Zhan, F. et al. (2003) Gene expression profiling of human plasma cell differentiation and classification of multiple myeloma based on similarities to distinct stages of late-stage B-cell development. Blood 101: 1128-1140
7. Kumar, S. et al. (2004) Bone marrow angiogenic ability and expression of angiogenic cytokines in myeloma: evidence favoring loss of marrow angiogenesis inhibitory activity with disease progression. Blood 104: 1159-1165
8. Lee, J. W. et al. (2004) IL-3 expression by myeloma cells increases both osteoclast formation and growth of myeloma cells. Blood 103: 2308-2315

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CD138 MicroBeads, human

For research use only

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Background information

Downstream applications

Columns

Figure 1

Selected references

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