CD105 MicroBeads were developed for the isolation of CD105
+
endothelial cells from single-cell suspensions of endothelial tissues, as well as for the isolation of mesenchymal stromal cells (MSCs) from bone marrow aspirate. CD105 MicroBeads can, for example, be used for the isolation of endothelial cells from:
umbilical cord veins,
arterial vessels1 ,
tumors2 ,
cultures of endothelial cells that are derived from synovial tissue.3
Background information
The CD105 antigen (endoglin) serves as a receptor for the growth and differentiation factors TGF-β
1
and TGF-β
3
, and is expressed on mature endothelial cells, on MSCs with mesodermal differentiation capacity, and on some leukemic cells of B lymphoid and myeloid origin. The CD105 MicroBeads recognize the same molecular antigen as anti-SH2 antibodies-the most commonly employed antigen for the definition of cultivated MSCs.
Downstream applications
CD105 MicroBeads can be used for studies on mesengenesis and for
in vitro
investigations on hematological disorders.
4
CD105
+
bone marrow cells also show multipotent differentiation
cells after 25 days in culture. Cells were isolated from the bone marrow aspirate of an acute myeloid leukemia patient.
4
Immunomagnetically selected CD105
+
cells after 25 days in culture. Cells were isolated from the bone marrow aspirate of an acute myeloid leukemia patient.
4
Selected references
Dentelli, P. et al. (2007) C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium. Blood 109: 4264-4271
Fonsato, V. et al. (2006) Expression of Pax2 in human renal tumor-derived endothelial cells sustains apoptosis resistance and angiogenesis. Am. J. Pathol. 168: 706-713
Santiago, B. et al. (2006) CXCL12 is displayed by rheumatoid endothelial cells through its basic amino-terminal motif on heparan sulfate proteoglycans. Arthritis Res. Ther. 8: R43
Campioni et al. (2003) MACS&more 7(1): 11-13
Majumdar, M. K. et al. (2000) Isolation, characterization, and chondrogenic potential of human bone marrow-derived multipotential stromal cells. J. Cell. Physiol. 185: 98-106
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