CD61 MicroBeads can be used for the direct isolation or depletion of megakaryocytes and their precursors from bone marrow aspirate as well as for the removal of platelets from peripheral blood cell preparations.
Background information
The CD61 antigen is also known as the integrin β3 subunit and platelet membrane glycoprotein IIIa (GPIIIa). CD61 and CD41 form the heterodimeric GPIIb/GPIIIa complex that is present on human megakaryocytes and platelets and mediates cell adhesion processes. Together with CD51, CD61 forms the vitronectin receptor on platelets as well as on a variety of other cell types, such as osteoclasts and vascular endothelial cells. CD61 MicroBeads were reported to cross-react with canine platelets.
Downstream applications
CD61
+
cells from bone marrow have been isolated for studies on megakaryocytopoiesis using the CD61 MicroBeads.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
megakaryocytes positively selected using MACS® Technology. (Courtesy of Dr. B. Schmitz, Cologne, Germany.)
CD61
+
megakaryocytes positively selected using MACS® Technology. (Courtesy of Dr. B. Schmitz, Cologne, Germany.)
Selected references
Schmitz et al. (1994) Magnetic activated cell sorting (MACS)--a new immunomagnetic method for megakaryocytic cell isolation: comparison of different separation techniques. Eur. J. Haematol. 52: 267-275
Ellis, W. J. et al. (2003) Detection and isolation of prostate cancer cells from peripheral blood and bone marrow. Urology 61: 277-281
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