The Anti-TCRγ/δ MicroBead Kit was developed for the positive selection or depletion of human T cells expressing the γ/δ variant of the T cell receptor (TCRγ/δ) from peripheral blood, lymph nodes, thymus, spleen, and cultured cells.
Background information
The Anti-TCRγ/δ MicroBead Kit consists of a hapten-conjugated Anti-TCRγ/δ antibody and FITC-conjugated Anti-Hapten MicroBeads. TCRγ/δ is expressed on up to 10% of the peripheral blood T cells.
Downstream applications
TCRγ/δ
+
T cells isolated with the Anti-TCRγ/δ MicroBead Kit have been expanded and used for proliferation and cytotoxicity assays.
1,2,3
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
cells from PBMCs using the Anti-TCRγ/δ MicroBead Kit, an MS Column, and a MiniMACS™ Separator.
Isolation and depletion of TCRγ/δ
+
cells from PBMCs using the Anti-TCRγ/δ MicroBead Kit, an MS Column, and a MiniMACS™ Separator.
Selected references
Schilbach, K. E. et al. (2000) Human gammadelta T lymphocytes exert natural and IL-2-induced cytotoxicity to neuroblastoma cells. J. Immunother. 23: 536-548
Schilbach, K. et al. (2001) Induction of proliferation and augmented cytotoxicity of gammadelta T lymphocytes by bisphosphonate clodronate. Blood 97: 2917-2918
Rothenfusser et al. (2001) Distinct CpG oligonucleotide sequences activate human gamma delta T cells via interferon-alpha/-beta. Eur. J. Immunol. 31: 3525-3534
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