Downstream applications

Columns

• Figure 1

  CD56 + cells were isolated from human peripheral blood mononuclear cells (PBMCs) using CD56 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD56-PE and CD3-APC and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  PBMCs before separation	Isolated CD56 + cells

• Selected references

  1. Fletcher, J. M. et al. (1998) Natural killer cell lysis of cytomegalovirus (CMV)-infected cells correlates with virally induced changes in cell surface lymphocyte function-associated antigen-3 (LFA-3) expression and not with the CMV-induced down-regulation of cell surface class I HLA. J. Immunol. 161: 2365-2374
  2. Doherty, D. G. et al. (1999)
     The human liver contains multiple populations of NK cells, T cells, and CD3

     ⁺

     CD56

     ⁺

     natural T cells with distinct cytotoxic activities and Tʜ1, Tʜ2, and Tʜ0 cytokine secretion patterns.
     J. Immunol. 163: 2314-2321
  3. Kumaki, S. et al. (2001) Identification of anti-herpes simplex virus antibody-producing B cells in a patient with an atypical RAG1 immunodeficiency. Blood 98: 1464-1468
  4. Xu, S. et al. (2006) Cardiac bodies: a novel culture method for enrichment of cardiomyocytes derived from human embryonic stem cells. Stem Cells Dev. 15: 631-639
  5. Sinanan, A. et al. (2004) Human adult craniofacial muscle-derived cells: neural-cell adhesion-molecule (NCAM; CD56)-expressing cells appear to contain multipotential stem cells. Biotechnol. Appl. Biochem. 40: 25-34
  6. De Luna, N. et al. (2006)
     Absence of dysferlin alters myogenin expression and delays human muscle differentiation

     in vitro

     .
     J. Biol. Chem. 281: 17092-17098

• Brochures and posters

  Natural killer cell research