YILIMART - CD43 (Ly-48) MicroBeads, mouse

CD43 (Ly-48) MicroBeads were developed for the isolation of untouched resting B cells (B-2 cells) from peripheral lymphoid tissues or late B cell progenitors from bone marrow.

Background information

The CD43 antigen is expressed on nearly all leukocytes except immature and mature resting B cells.

Detailed separation procedure

By using CD43 MicroBeads, all CD43-expressing cells are magnetically labeled, whereas the resting B cell subset stays untouched and is collected in the column effluent.

Downstream applications

Resting B cells isolated with CD43 MicroBeads have been used for various studies on B cell differentiation and related molecular processes. Resting B cells isolated from spleen were used for

in vitro

analyses on antibody class switching.

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They were separated for comparative analysis on B cell receptor signal transduction in wild type and immunodeficient mice

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or were used as starting material for studies on germinal center formation.

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Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Isolation of untouched B cells from a mouse spleen cell suspension using CD43 MicroBeads, a MiniMACS™ Separator, and an MS Column.

Spleen cells before separation	Isolated untouched B cells

Selected references
1. Hein, K. et al. (1998) Processing of switch transcripts is required for targeting of antibody class switch recombination. J. Exp. Med. 188: 2369-2374
2. Xu, S. et al. (2000) B cell development and activation defects resulting in xid-like immunodeficiency in BLNK/SLP-65-deficient mice. Int. Immunol. 12: 397-404
3. de Vinuesa, C. G. et al. (2000) Germinal centers without T cells. J. Exp. Med. 191: 485-493

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CD43 (Ly-48) MicroBeads, mouse

For research use only

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Background information

Detailed separation procedure

Downstream applications

Columns

Figure 1

Selected references

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