Mouse CD62L (L-selectin) MicroBeads were developed for the positive selection or depletion of T cell subsets from lymph nodes or spleen.
Background information
CD62L is highly expressed on naive T cells and down-regulated upon activation. It is also expressed on a small subset of memory T cells, the central memory T cells, which can be distinguished from naive T cells by their high expression of CD44. Furthermore, CD62L is expressed on most thymocytes, naive cytotoxic T cells, B cells, dendritic cells, macrophages, NK cells, neutrophils, and eosinophils.
For isolation of CD4
+
CD62L
+
naive T cells, the CD4
+
CD62L
+
T Cell Isolation Kit II is available.
Downstream applications
Naive T cells isolated following a MultiSort strategy were used for studies on cytokine expression,
1,2
receptor signaling,
3
and T cell activation by different antigen-presenting cells.
4
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
Two step isolation of naive and effector/memory T cells using a T cell-specific FITC-conjugated antibody, Anti-FITC-MultiSort Kit, and CD62L MicroBeads.
Two step isolation of naive and effector/memory T cells using a T cell-specific FITC-conjugated antibody, Anti-FITC-MultiSort Kit, and CD62L MicroBeads.
Selected references
Assenmacher, M. et al. (1998) Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4. J. Immunol. 161: 2825-2832
Assenmacher et al. (1998) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes. Eur. J. Immunol. 28: 1534-1543
Flynn, S. et al. (1998) CD4 T cell cytokine differentiation: the B cell activation molecule, OX40 ligand, instructs CD4 T cells to express interleukin 4 and upregulates expression of the chemokine receptor, Blr-1. J. Exp. Med. 188: 297-304
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