YILIMART - CD11b MicroBeads, human, mouse

CD11b MicroBeads were developed for the isolation or depletion of human and mouse cells based on their CD11b expression.

Background information

In humans, CD11b is strongly expressed on myeloid cells, and weakly expressed on NK cells and some activated lymphocytes.

In mice, the CD11b antigen is expressed on monocytes/macrophages and to a lower extent on granulocytes, NK cells, CD5

⁺

B1 cells, and a subset of dendritic cells.

Downstream applications

In humans, CD11b MicroBeads can be used for positive selection or depletion of CD11b

⁺

cells from PBMCs, bone marrow, and other lymphoid tissues. They are mostly used in combination with other MicroBeads for depletion of unwanted cells.

In mice, CD11b MicroBeads have been used to isolate CD11b

⁺

cells from cell suspensions of spleen

¹

, lymph node

²

, peritoneal cavity

³

, liver

⁴

, muscle tissue

²

, or blood

⁵

. They were also used in combination with other MicroBeads for the enrichment of mast cells from peritoneal cell suspensions.

³

For the isolation of human monocytes CD14 MicroBeads or the Pan Monocyte Isolation Kit are recommended.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Positive selection of murine CD11b

⁺

cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator

Mouse splenocytes before separation	CD11b-depleted splenocytes

Isolated CD11b ⁺ mouse cells

Figure 2
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Figure 2

Separation of CD11b
⁺
cells from human PBMCs using CD11b MicroBeads, an MS Column, and a MiniMACS™ Separator.

Separation of CD11b
⁺
cells from human PBMCs using CD11b MicroBeads, an MS Column, and a MiniMACS™ Separator.

Selected references
1. Lohoff, M. et al. (1997)
  
  Interferon regulatory factor-1 is required for a T helper 1 immune response
  in vivo
  .
  
  Immunity 6: 681-689
2. Dupuis, M. et al. (2000) Distribution of DNA vaccines determines their immunogenicity after intramuscular injection in mice. J. Immunol. 165: 2850-2858
3. Chen, X. J. et al. (1999) Surface and gene expression of immunoglobulin E receptors on mast cells and mast-cell numbers in interleukin-4-gene knockout mice. Immunology 96: 544-550
4. Do, H. et al. (1999) Expression of factor VIII by murine liver sinusoidal endothelial cells. J. Biol. Chem. 274: 19587-19592
5. Mathy, N. L. et al. (2000) Cutting edge: CD4 is not required for the functional activity of IL-16. J. Immunol. 164: 4429-4432
Brochures and posters

MACS Cell Pre-Enrichment for flow sorting

Human myeloid cells from peripheral blood

Isolation and analysis of murine myeloid cells from lymphoid tissues and tumor

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CD11b MicroBeads, human, mouse

For research use only

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Background information

Downstream applications

Columns

Figure 1

Figure 2

Figure 2

Selected references

Brochures and posters

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