Mouse CD45R (B220) MicroBeads were developed for the positive selection or depletion of mouse B cells from various lymphoid tissues like bone marrow, lymph nodes, and spleen or from non-lymphoid sources like lamina propria, peritoneal cavity, or lung.
Background information
The CD45R antigen is expressed on B lineage cells throughout their development from early pro-B cell stages on and is down-regulated upon terminal differentiation to plasma cells. CD45R is also expressed on plasmacytoid dendritic cells.
Downstream applications
B cells purified with CD45R MicroBeads have been used for studies on the mechanism and regulation of immunoglobulin gene rearrangement,
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for analyses of signal transduction,
2,3
and for reconstitution of immunodeficient and parasite-infected mice.
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Columns
For positive selection: MS, LS, XS, or autoMACS
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Columns. For depletion: LD, D, or autoMACS Columns.
Isolation of B cells from mouse spleen cell suspension using CD45R MicroBeads, a MiniMACS™ Separator, and an MS Column.
Isolation of B cells from mouse spleen cell suspension using CD45R MicroBeads, a MiniMACS™ Separator, and an MS Column.
Selected references
Maki, K. et al. (2000) Immunoglobulin beta signaling regulates locus accessibility for ordered immunoglobulin gene rearrangements. J. Exp. Med. 191: 1333-1340
Weintraub, B. C. et al. (2000) Entry of B cell receptor into signaling domains is inhibited in tolerant B cells. J. Exp. Med. 191: 1443-1448
Suzuki, H. et al. (1999) Xid-like immunodeficiency in mice with disruption of the p85alpha subunit of phosphoinositide 3-kinase. Science 283: 390-392
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