Mouse CD5 (Ly-1) MicroBeads can be used for the positive selection or depletion of T cells or CD5
+
B-1a cells from peripheral lymphoid organs, non-lymphoid tissue or blood.
Background information
The CD5 antigen is highly expressed on T cells. CD5 is also expressed on B-1a cells but not on conventional B-2 cells. B-1 cells differ from B-2 cells with regard to origin, development, and tissue distribution and show an antigen-restricted B cell receptor repertoire.
Applications
CD5 MicroBeads can also be used for the positive selection of T cells and subsequent analysis of cytokine secretion kinetics during T cell differentiation.
For the isolation of highly pure B-1a cells by positive selection using CD5 MicroBeads, T cells have to be depleted in advance by using CD90.2 MicroBeads.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
cells from a mouse spleen cell suspension using CD5 (Ly-1) MicroBeads, a MiniMACS™ Separator, and an MS Column.
Positive selection of CD5
+
cells from a mouse spleen cell suspension using CD5 (Ly-1) MicroBeads, a MiniMACS™ Separator, and an MS Column.
Selected references
Hoerauf, A. et al. (1994) The Xid defect determines an improved clinical course of murine leishmaniasis in susceptible mice. Int. Immunol. 6: 1117-1124
Leitges, M. et al. (1996) Immunodeficiency in protein kinase cbeta-deficient mice. Science 273: 788-791
Taki, S. et al. (1997) Multistage regulation of Tʜ1-type immune responses by the transcription factor IRF-1. Immunity 6: 673-679
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