Anti-Rabbit IgG MicroBeads, formerly known as Goat Anti-Rabbit IgG MicroBeads, can be used for magnetic labeling and subsequent separation of cells or other target materials labeled with rabbit antiserum. Anti-Rabbit IgG MicroBeads react with the heavy chains of all rabbit IgG antibodies. These MicroBeads are conjugated with polyclonal goat F(ab')
2
fragments which have been affinity-adsorbed to remove cross-reactivity to human immunoglobulins.
Downstream applications
The versatility of Anti-Rabbit IgG MicroBeads was shown by the isolation of murine CD34
+
progenitor cells
1
and the isolation of TGFα
+
cells from human bone marrow
2
. Anti-Rabbit IgG MicroBeads have also been used for the isolation of
Cryptosporidium parvum
oocysts from spiked samples
3
and for the enrichment of spermatogonia from Djungarian hamster, mouse, and marmoset monkey testes
4
.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
Separation of mouse spleen cells using a FITC-conjugated rabbit anti-mouse IgG antibody, Anti-Rabbit IgG MicroBeads, and a MiniMACS™ Separator with an MS Column.
Separation of mouse spleen cells using a FITC-conjugated rabbit anti-mouse IgG antibody, Anti-Rabbit IgG MicroBeads, and a MiniMACS™ Separator with an MS Column.
Selected references
Cheng et al. (1996) Hematopoietic defects in mice lacking the sialomucin CD34. Blood 87: 479-490
Walz et al. (1995) Blood 9: 2385-2392
Deng, M. Q. et al. (2000) A sensitive and specific tritium release assay for dopamine-beta-hydroxylase (DbetaH) in serum. J. Microbiol. Methods 40: 11-17
von Schönfeldt, V. et al. (1999) Magnetic cell sorting is a fast and effective method of enriching viable spermatogonia from Djungarian hamster, mouse, and marmoset monkey testes. Biol. Reprod. 61: 582-589
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