Anti-Rat IgG MicroBeads, formerly known as Goat Anti-Rat IgG MicroBeads, can be used in combination with any primary rat IgG antibody for the magnetic labeling and separation of cells. Anti-Rat IgG MicroBeads recognize the heavy and light chains of all rat IgG isotypes. The goat F(ab')
2
fragments have been affinity adsorbed to remove any cross-reactivity to mouse immunoglobulins.
Downstream applications
Anti-Rat IgG MicroBeads have been used for the isolation of murine leukocyte subsets from brain tissue
1
, for the separation of murine T cell subsets from PBMCs
2
, for the isolation of murine dermal macrophages
3
as well as for the isolation of intraepithelial CD8β
+
lymphocytes from murine intestine
4
. They have also been used for the separation of CD105- and CD106-expressing endothelial cells from murine aortic tissue.
5
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
Separation of mouse spleen cells using rat anti-mouse CD45R antibody (isotype: IgG2a), Anti-Rat IgG MicroBeads, and a MiniMACS™ Separator with an MS Column.
Separation of mouse spleen cells using rat anti-mouse CD45R antibody (isotype: IgG2a), Anti-Rat IgG MicroBeads, and a MiniMACS™ Separator with an MS Column.
Selected references
Schlüter et al. (1996) Am. J. Pathol. 150: 1021-1035
Murine psoriasis-like disorder induced by naive CD4
+
T cells.
Nat. Med. 3: 183-188
Sato, K. et al. (1998) Contribution of dermal macrophage trafficking in the sensitization phase of contact hypersensitivity. J. Immunol. 161: 6835-6844
Lepage, A. C. et al. (1998) Gut-derived intraepithelial lymphocytes induce long term immunity against Toxoplasma gondii. J. Immunol. 168: 4902-4908
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