Anti-Mouse IgG MicroBeads, formerly known as Goat Anti-Mouse IgG MicroBeads, recognize the heavy and light chains of all mouse IgG antibodies (IgG1, IgG2a, IgG2b, and IgG3). They also react with light chains of other mouse immunoglobulin classes like IgM, IgA, or IgE. Anti-Mouse IgG MicroBeads are conjugated with goat F(ab')
2
fragments which have been affinity-adsorbed to remove cross-reactivity with human immunoglobulins.
Downstream applications
Anti-Mouse IgG MicroBeads have been used for numerous different cell separations, including tumor cells from patients blood
1,2
, fetal trophoblasts from maternal blood
3
, human T cell subsets
4
, and human NK cells
5
from PBMCs as well as the isolation of chicken osteocytes from chicken calvariae and tibiotarsi. Positive selection of trout thrombocytes
6
, a subpopulation of rat macrophages
7
, and porcine hematopoietic stem cells
8
with Anti-Mouse IgG MicroBeads has been reported, as well as the depletion of B cells from
Xenopus laevis
spleen
9
.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
Chicken osteocytes isolated from chicken calvariae and tibiotarsi using mAb 7.3 and Anti-Mouse IgG MicroBeads. (Courtesy of Dr. Suswillo, London, UK.)
Chicken osteocytes isolated from chicken calvariae and tibiotarsi using mAb 7.3 and Anti-Mouse IgG MicroBeads. (Courtesy of Dr. Suswillo, London, UK.)
Selected references
Wong et al. (1995) Detection of circulating tumour cells with the magnetic activated cell sorter. Br. J. Surg. 82: 1333-1337
Benez, A. et al. (1999) Detection of circulating melanoma cells by immunomagnetic cell sorting. J. Clin. Lab. Anal. 13: 229-233
van Wijk, I.J. et al. (1996) Enrichment of fetal trophoblast cells from the maternal peripheral blood followed by detection of fetal deoxyribonucleic acid with a nested X/Y polymerase chain reaction. Am. J. Obstet. Gynecol. 174: 871-876
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