YILIMART - Streptavidin MicroBeads (1 ml)

Streptavidin MicroBeads are used for the indirect magnetic labeling and separation of cells, subcellular material, or bacteria labeled with a biotinylated primary antibody or ligand.

Applications

Streptavidin MicroBeads have been used for the isolation of human allergen-specific B cells

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or follicular dendritic cells

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, murine liver sinusoidal endothelial cells

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, sheep B cell subpopulations

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, and rat retinal ganglion cells

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. They have also been used in many other separations such as the isolation of plant protoplasts.

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Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Potato protoplasts isolated by MACS

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Technology using biotinylated lectins and Streptavidin MicroBeads. (Courtesy of Dr. Dörr, Cologne, Germany.)

Positively selected protoplasts	Negative fraction

Figure 2
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Figure 2

Follicular dendritic cells isolated by MACS
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Technology from human tonsils using Streptavidin MicroBeads. (Courtesy of Dr. J. Schmitz, Hamburg, Germany.)

Follicular dendritic cells isolated by MACS
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Technology from human tonsils using Streptavidin MicroBeads. (Courtesy of Dr. J. Schmitz, Hamburg, Germany.)

Selected references
1. Zürcher, A. W. et al. (1995) IgE-producing hybridomas established after B-cell culture in the CD40 system. Immunol. Lett. 46: 49-57
2. Schmitz, J. et al. (1993) Optimizing follicular dendritic cell isolation by discontinuous gradient centrifugation and use of the magnetic cell sorter (MACS). J. Immunol. Methods 159: 189-196
3. Do, H. et al. (1999) Expression of factor VIII by murine liver sinusoidal endothelial cells. J. Biol. Chem. 274: 19587-19592
4. Gupta et al. (1998) Two B cell subpopulations have distinct recirculation characteristics. Eur. J. Immunol. 28: 1597-1603
5. Shoge, K. et al. (1999) Rat retinal ganglion cells culture enriched with the magnetic cell sorter. Neurosci. Lett. 259: 111-114
6. Dörr et al. (1994) Biotechnology 12: 511-515

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Streptavidin MicroBeads (1 ml)

For research use only

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Applications

Columns

Figure 1

Figure 2

Figure 2

Selected references

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