Anti-Rat Kappa MicroBeads were developed for the positive selection or depletion of cells labeled with primary rat antibodies carrying kappa light chains. They can also be used for the positive selection or depletion of rat B cells according to the expression of the immunoglobulin (Ig) kappa light chain.
Background information
A majority (95%) of all rat B cells express the Igκ light chain. Anti-Rat Kappa MicroBeads bind equally well to the Ig light chain allotypes Igκ-1a and Igκ-1b. Together with the Ig heavy chain, the Ig light chain is expressed on the surface of immature IgM
+
IgD
-
B cells. Furthermore, it is present on mature IgM
+
IgD
+
B cells and memory B cells, and is down-regulated on activated B cells upon terminal differentiation into plasma cells.
Downstream applications
B cells isolated with Anti-Rat Kappa MicroBeads from peripheral lymphoid organs or non-hematopoietic tissues are suitable for
in vivo
and
in vitro
studies on B cell development, differentiation, and migration. Highly pure B cell populations are also required for reliable cellular and molecular analyses, for example, of Ig gene rearrangement.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
Separation of CD4+ cells from mouse spleen using a rat anti-mouse CD4 antibody with kappa light chains (isotype IgG2b), Anti-Rat Kappa MicroBeads, and an MS Column.
Separation of CD4+ cells from mouse spleen using a rat anti-mouse CD4 antibody with kappa light chains (isotype IgG2b), Anti-Rat Kappa MicroBeads, and an MS Column.
Selected references
Diacovo, T. G. et al. (1996) Interactions of human alpha/beta and gamma/delta T lymphocyte subsets in shear flow with E-selectin and P-selectin. J. Exp. Med. 183: 1193-1203
Svensson, M. et al. (1997) Bone marrow-derived dendritic cells can process bacteria for MHC-I and MHC-II presentation to T cells. J. Immunol. 158: 4229-4236
Fuhlbrigge, R. C. et al. (1997) Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 389: 978-981
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