YILIMART - Anti-Mouse IgM-Microbeads, 1 ml

Anti-Mouse IgM MicroBeads are suitable for magnetic labeling and subsequent separation of cells where the primary antibody is of the mouse IgM isotype. They can also be used for direct positive selection or depletion of mouse B cells expressing IgM on the surface.

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Applications

Anti-Mouse IgM MicroBeads have been used for the isolation of lymphocyte subsets

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, for the isolation of murine primordial germ cells

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as well as for the isolation of follicular dendritic cells from human tonsils

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Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Separation of CD16

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cells from human peripheral blood leukocytes using a mouse anti-human CD16 antibody (isotype IgM), Anti-Mouse IgM MicroBeads, and an MS Column.

Unseparated fraction	Positive fraction

Selected references
1. Pelanda, R. et al. (1996) A prematurely expressed Ig(kappa) transgene, but not V(kappa)J(kappa) gene segment targeted into the Ig(kappa) locus, can rescue B cell development in lambda5-deficient mice. Immunity 5: 229-239
2. Underhill et al. (2002) IgG plasma cells display a unique spectrum of leukocyte adhesion and homing molecules. Blood : 2905-2912
3. Diacovo, T. G. et al. (1996) Interactions of human alpha/beta and gamma/delta T lymphocyte subsets in shear flow with E-selectin and P-selectin. J. Exp. Med. 183: 1193-1203
4. Pesce and De Felici (1995) Purification of mouse primordial germ cells by MiniMACS magnetic separation system. Dev. Biol. 170: 722-725
5. Butch, A. W. et al. (1999) Human follicular dendritic cells inhibit superantigen-induced T-cell proliferation by distinct mechanisms. Blood 94: 216-224
Special protocols

Positive Selection of Antibody Expressing Hybridoma Cells

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Anti-Mouse IgM-Microbeads, 1 ml

For research use only

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Applications

Columns

Figure 1

Selected references

Special protocols

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