Anti-Mouse IgG2a+b MicroBeads can be used for magnetic labeling and subsequent separation of cells where the primary antibody is of the mouse IgG2a or IgG2b isotype. They can also be used for direct positive selection or depletion of mouse B cells expressing IgG2a or IgG2b on their surface.
Applications
Anti-Mouse IgG2a+b MicroBeads have been utilized for the separation of human T cell subsets
1
as well as for the enrichment of fetal erythrocytes
2
, epithelial tumor cells
3
, or bovine leukocytes
4
.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
Separation of monocytes from human PBMCs using a mouse anti-human CD14 antibody (isotype: IgG2a), Anti-Mouse IgG2a+b MicroBeads, and a MiniMACS™ Separator with an MS Column.
Separation of monocytes from human PBMCs using a mouse anti-human CD14 antibody (isotype: IgG2a), Anti-Mouse IgG2a+b MicroBeads, and a MiniMACS™ Separator with an MS Column.
Selected references
Jung, T. et al. (1993) Detection of intracellular cytokines by flow cytometry. J. Immunol. Methods 159: 197-207
Zheng, Y. L. et al. (1993) Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting. J. Med. Genet. 30: 1051-1056
Griwatz, C. et al. (1995) An immunological enrichment method for epithelial cells from peripheral blood. J. Immunol. Methods 183: 251-265
Brooke et al. (1998) Cloning of two members of the SIRP alpha family of protein tyrosine phosphatase binding proteins in cattle that are expressed on monocytes and a subpopulation of dendritic cells and which mediate binding to CD4 T cells. Eur. J. Immunol. 28: 1-11
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