YILIMART - Anti-Mouse IgG1-Microbeads, 2 ml

Anti-Mouse IgG1 MicroBeads can be used for magnetic labeling and subsequent separation of cells, which are labeled with a primary mouse IgG1 antibody. They can also be used for positive selection or depletion of mouse B cells expressing IgG1 on the cell surface.

Downstream applications

Anti-Mouse IgG1 MicroBeads have been used for the isolation of T cells and T cell subpopulations

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, B cell subsets

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, rat type I alveolar epithelial cells

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, and for the isolation of rare cells, such as subsets of kidney cells

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, ovarian carcinoma cells

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, or myeloma cells

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. The isolation of keratinocytes by depletion of non-keratinocytes has also been performed with Anti-Mouse IgG1 MicroBeads.

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Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Human kidney cell subsets isolated by using an anti-Tamm-Horsefall glycoprotein antibody for distal tubulus cells and a CD13 antibody for proximal tubulus cells in combination with Anti-Mouse IgG1 MicroBeads. (Courtesy of Dr. Baer, Frankfurt a.M., Germany.)

Kidney cells before MACS® Separation	Purified distal tubulus cells

Purified proximal tubulus cells

Selected references
1. Jung, T. et al. (1993) Detection of intracellular cytokines by flow cytometry. J. Immunol. Methods 159: 197-207
2. Näsman et al. (1996) Evidence for oligoclonal diversification of the VH6-containing immunoglobulin repertoire during reconstitution after bone marrow transplantation. Blood 87: 2795-2804
3. Dobbs, L. G. et al. (1998) Highly water-permeable type I alveolar epithelial cells confer high water permeability between the airspace and vasculature in rat lung. Proc. Natl. Acad. Sci. U.S.A. 95: 2991-2996
4. Baer, P. C. et al. (1997) Isolation of proximal and distal tubule cells from human kidney by immunomagnetic separation. Technical note. Kidney Int. 52: 1321-1331
5. Harbeck, N. et al. (1995) Model system for isolation of competent ovarian-carcinoma cells from fresh tumor-tissue by a magnetic separation technique (MACS). Int. J. Oncol. 6: 1249-1254
6. Drach et al. (1995)
  
  Multiple myeloma: high incidence of chromosomal aneuploidy as detected by interphase fluorescence
  in situ
  hybridization.
  
  Cancer Res. 55: 3854-3859
7. Formanek et al. (1998) Magnetic cell separation for purification of human oral keratinocytes: an effective method for functional studies without prior cell subcultivation. Eur. Arch. Otorhinolaryngol. 255: 211-215
Special protocols

Positive Selection of Antibody Expressing Hybridoma Cells

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Anti-Mouse IgG1-Microbeads, 2 ml

For research use only

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Downstream applications

Columns

Figure 1

Selected references

Special protocols

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