Background information

Downstream applications

Columns

• Figure 1

  Flow cytometric analysis of CD34 + cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.

  BM-MNCs before separation	Isolated CD34 + cells

• Selected references

  1. Brault, J. et al. (2014)
     Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:

     ex vivo

     models of X

     ⁰

     -linked, AR22

     ⁰

     - and AR47

     ⁰

     - chronic granulomatous diseases.
     Biores Open Access 3(6): 311-326
  2. Schreiber, T. D. et al. (2009) The integrin alpha9beta1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation. Haematologica 94: 1493-1501
  3. Jing et al. (2010) CD49d blockade by natalizumab in patients with multiple sclerosis affects steady-state hematopoiesis and mobilizes progenitors with a distinct phenotype and function. Bone Marrow Transplant. 45: 1489-1496
  4. Ban, H. et al. (2011) Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc. Natl. Acad. Sci. U.S.A. 108: 14234-9
  5. Bandi, S. and Akkina, R. (2008) Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection. AIDS Res. Ther. 5: 1
  6. Wang, Z. Z. et al. (2007) Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat. Biotechnol. 25: 317-318
  7. Loges, S. et al. (2004) Identification of the adult human hemangioblast. Stem Cells Dev. 13: 229-242
  8. Peichev, M. et al. (2000)
     Expression of VEGFR-2 and AC133 by circulating human CD34

     ⁺

     cells identifies a population of functional endothelial precursors.
     Blood 95: 952-958
  9. Rafii, S. and Lyden, D. (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat. Med. 9: 702-712
  10. Valgimigli, M. et al. (2004)
      CD34

      ⁺

      and endothelial progenitor cells in patients with various degrees of congestive heart failure.
      Circulation 110: 1209-1212
  11. Chen et al. (2011) Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice. Blood 117: 1530-1539

• Customer reports and application notes

  Array-based analysis of microRNA and mRNA expression characterizes early differentiation pathways of CD133

  ⁺

  and CD34

  ⁺

  hematopoietic stem and progenitor cells. Bissels et al. (2012) MACS&more 14(2).