YILIMART - Indirect CD34 MicroBead Kit, human

Indirect CD34 MicroBead Kit, human

For research use only

货号：: 130-046-701
描述：: Indirect CD34 MicroBead Kit, human
包装：: for 2×10⁹ total cells

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The Indirect CD34 MicroBead Kit was developed for the isolation of CD34

⁺

precursor cells, using a two-step procedure involving indirect magnetic labeling from

peripheral blood
bone marrow
leukapheresis product
cord blood
differentiated-ES and iPS cells

Even faster, one-step isolation of CD34

⁺

cells can be achieved with the CD34 MicroBead Kit.

Background information

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts

^1,2

, endothelial progenitor cells

^1,3-5

, and mature endothelial cells

⁴

.

Detailed separation procedure

First, cells are labeled with the hapten-modified CD34 primary antibody QBEND/10

⁶

. In a second step, antibody-labeled cells are magnetically labeled with Anti-Hapten MicroBeads.

Control staining of cells isolated with the Indirect CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

Transplantation of CD34⁺ cells into humanized mouse models
Expansion and differentiation in culture
Reprogramming of CD34⁺ HSCs into iPS cells
Differentiation of ES or iPS cell-derived CD34⁺ -derived precursors into endothelial or hematopoietic lineages
Evaluation of differentiation potential in CFU assays
Characterization of surface marker expression by flow cytometry.

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Figure 1
View details

Figure 1

May-Grünwald-Giemsa–stained CD34
⁺
cells isolated from bone marrow aspirate using the Indirect CD34 MicroBead Kit, an MS Column and a MiniMACS™ Separator.

May-Grünwald-Giemsa–stained CD34
⁺
cells isolated from bone marrow aspirate using the Indirect CD34 MicroBead Kit, an MS Column and a MiniMACS™ Separator.

Selected references
1. Wang, Z. Z. et al. (2007) Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat. Biotechnol. 25: 317-318
2. Loges, S. et al. (2004) Identification of the adult human hemangioblast. Stem Cells Dev. 13: 229-242
3. Peichev, M. et al. (2000)
  
  Expression of VEGFR-2 and AC133 by circulating human CD34
  ⁺
  cells identifies a population of functional endothelial precursors.
  
  Blood 95: 952-958
4. Rafii, S. and Lyden, D. (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat. Med. 9: 702-712
5. Valgimigli, M. et al. (2004)
  
  CD34
  ⁺
  and endothelial progenitor cells in patients with various degrees of congestive heart failure.
  
  Circulation 110: 1209-1212
6. Lanza, F. et al. (2001) Structural and functional features of the CD34 antigen: an update. J. Biol. Regul. Homeost. Agents 15: 1-13

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