YILIMART - CD71 MicroBeads, human

CD71 MicroBeads are developed for the positive selection or depletion of CD71-expressing cells from peripheral blood mononuclear cells (PBMCs), cord blood, bone marrow, or lymphoid tissue.

Background information

The CD71 antigen is also known as the transferrin receptor, which is essential for iron transport into proliferating cells. It is expressed on mesenchymal stromal cells (MSCs) in bone marrow

¹

, on activated T and B lymphocytes, macrophages, and on all proliferating cells. It is up-regulated on lymphocytes during proliferative responses to antigens or mitogens but is not expressed on resting lymphocytes. Furthermore, CD71 is present on reticulocytes and erythroid progenitors in fetal liver, cord blood, and peripheral blood, but is downregulated as these cells differentiate into mature erythrocytes

^2–4

.

Downstream applications

Enrichment of fetal early erythroid cells from maternal peripheral blood^5,6
Isolation of hematopoietic precursor cells committed to the erythroid lineage (BFU-E) when used in combination with the CD34 MultiSort Kit

Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Separation of CD71

⁺

cells from cord blood mononuclear cells (CB-MNCs) using CD71 MicroBeads and the Possel separation program on the autoMACS® Separator. Cells were fluorescently stained with CD71-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

CB-MNCs before separation	CD71 ⁺ cells (reticulocytes)

Figure 2
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Figure 2

Isolation of CD71
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cells from maternal blood after depletion of leukocytes and monocytes using CD45 and CD14 MicroBeads. (Courtesy of Dr. Jürgen Büsch, Cologne, Germany.)

Isolation of CD71
⁺
cells from maternal blood after depletion of leukocytes and monocytes using CD45 and CD14 MicroBeads. (Courtesy of Dr. Jürgen Büsch, Cologne, Germany.)
Figure 3
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Figure 3

Giemsa-stained reticulocytes isolated from human buffy coat using CD71 MicroBeads.

Giemsa-stained reticulocytes isolated from human buffy coat using CD71 MicroBeads.

Selected references
1. Pittenger, M. F. et al. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147
2. Judd, W. et al. (1980) Novel surface antigen expressed on dividing cells but absent from nondividing cells. J. Exp. Med. 152: 1430-1435
3. Loken, M. R. et al. (1987) Flow cytometric analysis of human bone marrow: I. Normal erythroid development. Blood 69: 255-263
4. Phillips, J. H. et al. (1984) Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens. J. Exp. Med. 159: 993-1008
5. Büsch, J. et al. (1994) Enrichment of fetal cells from maternal blood by high gradient magnetic cell sorting (double MACS) for PCR-based genetic analysis. Prenat. Diagn. 14: 1129-1140
6. Cheung, M. C. et al. (1996) Prenatal diagnosis of sickle cell anaemia and thalassaemia by analysis of fetal cells in maternal blood. Nat. Genet. 14: 264-268

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CD71 MicroBeads, human

For research use only

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Background information

Downstream applications

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Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Selected references

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