Interleukin 2 (IL-2), a potent lymphoid cell growth factor, plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes, for instance, proliferation and differentiation of T cells, NK cells, and B cells.
MACS GMP Recombinant Human IL-2 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
IL-2 is a typical four α-helix bundle cytokine and is produced by activated T cells, especially the CD4
+
T helper cell population. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β-chain, and common γ-chain.
Applications
MACS GMP Recombinant Human IL-2 can be used for a variety of applications, including the
ex vivo
activation and expansion of T cells, e.g., antigen-specific cytotoxic T lymphocytes
2,3
or regulatory T cells
4
or the
ex vivo
stimulation of NK cells
5,6
.
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay according to Gearing and Bird
1
using CTLL-2 cells. The proliferation assay was calibrated with the 2nd international standard for human IL-2 (NIBSC code 86/500) provided by the National Institute for Biological Standards and Control.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-2. The peak corresponds to the calculated molecular mass of 15400 Da.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-2. The peak corresponds to the calculated molecular mass of 15400 Da.
Selected references
Gearing, A. J. H. and Bird, C. B. (1987) Lymphokines and interferons, a practical approach. Oxford: IRL Press : 295
Zhang, H. et al. (2007) 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. J. Immunol. 179: 4910-4918
-expanded human natural killer cells up-regulate activating receptors and death receptor ligands and have enhanced cytolytic activity against tumor cells.
Cytotherapy 11: 341-355
McKenna, D. H. Jr. et al. (2007) Good manufacturing practices production of natural killer cells for immunotherapy: a six-year single-institution experience. Transfusion 47(3): 520-528
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