Interleukin 7 (IL-7) is a pleiotropic cytokine with central roles in modulating T cell development and peripheral T cell homeostasis. IL-7 can act both as a T cell growth factor as well as a critical anti-apoptotic survival factor for naive and memory T cells.
MACS GMP Recombinant Human IL-7 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
The primary sources of IL-7 are non-hematopoietic stromal cells in bone marrow, thymus, and lymphoid organs and tissues. IL-7 is a member of the type I cytokine family. It is related to IL-2 and signals through a heterodimeric receptor composed of the common cytokine signaling γ-chain and IL-7 receptor α-chain.
Applications
MACS GMP Recombinant Human IL-7 can be used for a variety of applications, including the
ex vivo
generation of antigen-specific cytotoxic T lymphocytes.
2,3
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay according to Ishihara
et al.1
using mouse 2E8 cells. The proliferation assay was calibrated with the reference standard for human IL-7 (NIBSC code 90/530) provided by the National Institute for Biological Standards and Control.
Activity of Human IL-7, GMP grade (blue bar) was compared to another commercially available product (black bar).
Human IL-7 biological activity.
Activity of Human IL-7, GMP grade (blue bar) was compared to another commercially available product (black bar).
Selected references
Ishihara, K. et al. (1991) Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B-to B-cell transition. Dev. Immunol. 1: 149-161
Zhang, H. et al. (2007) 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. J. Immunol. 179: 4910-4918
Kaneko et al. (2009) IL-7 and IL-15 allow the generation of suicide gene-modified alloreactive self-renewing central memory human T lymphocytes. Blood 113: 1006-1015
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