Interleukin 4 (IL-4) is an anti-inflammatory cytokine and is a key regulator in humoral and adaptive immunity. It has complex biological roles and promotes growth and differentiation of B cells and T cells, and cells of the monocytic lineage.
MACS GMP Recombinant Human IL-4 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
IL-4 is predominantly secreted by activated CD4
+
memory and effector T
H
2 cells, basophils, and mast cells. It promotes the proliferation and differentiation of B cells, as well as immunoglobulin isotype switching, and MHC class II antigen and low-affinity IgE receptor expression. Furthermore, IL-4 induces the differentiation of naive CD4
+
T cells into helper T
H
2 cells, while suppressing T
H
1 development, and promotes chemotaxis of mast cells and basophils.
Applications
MACS GMP Recombinant Human IL-4 can be used for a variety of applications, including the
ex vivo
generation of human dendritic cells from enriched CD14
+
monocyte populations.
2–4
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay according to Kitamura
et al.1
using TF-1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human IL-4 (NIBSC code 88/656) provided by the National Institute for Biological Standards and Control.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-4. The peak corresponds to the calculated molecular mass of 15088 Da.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-4. The peak corresponds to the calculated molecular mass of 15088 Da.
Selected references
Kitamura, T. et al. (1991) IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoetic cell line, TF-1. Int. Immunol. 3: 571-577
Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
Jonuleit et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142
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